Human Gut Microbiome and Body MetabolismWord文件下载.docx
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Human Gut Microbiome and Body MetabolismWord文件下载.docx
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PeeraHemarajata,1,2
and
JamesVersalovic1,2,*
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Abstract
BACKGROUND
Obesity,metabolicsyndrome,andtype2diabetesaremajorpublichealthchallenges.Recently,interesthassurgedregardingthepossibleroleoftheintestinalmicrobiotaaspotentialnovelcontributorstotheincreasedprevalenceofthese3disorders.
CONTENT
RecentadvancesinmicrobialDNAsequencingtechnologieshaveresultedinthewidespreadapplicationofwhole-genomesequencingtechnologiesformetagenomicDNAanalysisofcomplexecosystemssuchasthehumangut.Currentevidencesuggeststhatthegutmicrobiotaaffectnutrientacquisition,energyharvest,andamyriadofhostmetabolicpathways.
CONCLUSION
AdvancesintheHumanMicrobiomeProjectandhumanmetagenomicsresearchwillleadthewaytowardagreaterunderstandingoftheimportanceandroleofthegutmicrobiomeinmetabolicdisorderssuchasobesity,metabolicsyndrome,anddiabetes.
Obesity,metabolicsyndrome,andtype2diabetesaremajorpublichealthchallenges,affectingapproximately26millionchildrenandadultsintheUS.Morethan8%oftheUSpopulationhasdiabetes,ofwhich17.9millionpeoplehavethemetabolicsyndrome
(1).Duringthepast20years,obesityhasdramaticallyincreasedinprevalenceintheUS.Morethan1in3USadults(36%)areobese,andapproximately12.5million(17%)ofchildrenandadolescents(age2–19years)areobese
(2).IntheUSin2010
(2),allofthestateshadaprevalenceofobesityofover20%.Theheterogeneityofthesedisordershasbeendemonstratedthroughbothanthropometricandgeneticstudies.Thesemetabolicdisordersarebelievedtobecausedbyacombinationofgeneticsusceptibilitiesandlifestylechanges.Recently,interesthassurgedinthepossibleroleoftheintestinalmicrobiomeasapotentialcontributortotherapidlyincreasedprevalenceofobesity
(3–5).Thisreviewfocusesonrecentadvancesintheunderstandingofthegutmicrobiomeandtechniquestoassessthemicrobiomeanditsrelationshiptohumanbodymetabolism,obesity,metabolicsyndrome,andtype2diabetes
(Fig.1).
Hyperglycemia(HG)andincreasedfreefattyacids(FFA),whicharehallmarksofobesity,metabolicsyndrome,anddiabetes,combinedwithahigh-fat,high–glycemicloaddiet,couldresultinincreasedactivationoftheinflammasomecomplexaswell
...
TheHumanGutMicrobiome:
TheToolkitbehindtheScience
Thewidespreadapplicationof16SrRNAgenesequencingfordetectionofbacterialpathogensandmicrobialecologyhasprovidedarobusttechnicalplatformfortheevaluationofthebacterialcompositionofthehumanmicrobiome.Sequencingof2primarytargetswithinbacterial16SrRNAgenesyieldedvaluablecompositionaldatapertainingtothehumanfecalmicrobiomeof242healthyadults
(6,
7).IntheHumanMicrobiomeProject,18differentbodysitesweresampledandsequenced.Stoolspecimenswerethesinglespecimentypeusedtostudytheintestinalmicrobiome.Previouslypublishedstudiesdemonstratedthevariationincompositionofthegutmicrobiomeamonglocationswithinthegastrointestinaltractindifferentmammalianspecies.Forexample,16SrRNAgenesequencinghasbeendeployedtostudythematurationofmurinececalmicrobiota,andthesestudiesdemonstratedtheexistenceofalargenumberofyet-unidentifiedbacteriathatinhabitthemammalianintestine
(6).Suchsequencingstrategies,whicharecultureindependent,areessentialfordeterminingbacterialcompositionofthemicrobiomeanditsrelativestabilityanddiversityovertime.Thus,itisessentialtodeveloprobustexperimentalmodelsofthehumanmicrobiometodelineateimportantmechanisticprocessesinthedevelopmentofhumandiseasestates.
Advancesinsequencingtechnologieshaveresultedinthewidespreadapplicationofwhole-genome(WG)3sequencingtechnologiesformetagenomicDNAanalysisofcomplexecosystemssuchasthehumanintestine
(7).WGsequencingstrategiesprovidemicrobialcompositionalaswellasfunctionalinformation.WGdatacanbeusedtoinferbacterialcomposition,andthesedatayieldinformationsimilartothatgeneratedby16SrRNAgenesequencing.Thegenomesequencesofhighlyabundantspeciesarewellrepresentedinasetofrandomshotgunreads,whereaslessabundantspeciesarerepresentedbyfewersequencesgeneratedinanext-generationsequencingrun.Thisrelativerichnesspermitsthecomprehensivemeasurementofthecompositionalresponsesofanecosystemtodietarychanges,drugtherapy,epigeneticalterations,andenvironmentalperturbations.Alternatively,mostgenes(usuallyapproximately2000genesperbacterium)inthemicrobiomearesequencedsothatmetabolicandotherfunctionalpathwayscanbeevaluatedineachindividual’smetagenome.FunctionalWGdataprovideopportunitiestofindoutwhichmetabolicpathwaysareaffectedandhowthemicrobiomemaycontributemechanisticallytohealthanddiseasestates.Thistechnologycreatestheformidablechallengeofmanagingvastdatasets.Advancesinnext-generationDNAsequencingyielded576.7GbofmicrobialDNAsequencedata,whichweregeneratedwithanIllumina™genomeanalyzer(Illumina)fromtotalDNAfromthestoolsamplesof124Europeanadults
(8).Therelationshipbetweenthecommensalmicrobiotathatcomprisethegutmicrobiotaandthosethatareintheintestinalbarrieriscomplexanddiffersspatiallythroughoutdifferentareasofthegastrointestinaltract.Fecalmetagenomicsmeasuresecosystemchangesinstoolorthedistalintestine,butitdoesnotcomparethemicrobiomesindifferentregionsoftheintestine.Itisalsoimportanttonotethatmetagenomicanalysisoffecalsamplesdoesnotincludeallimportantmolecularinteractionswithinthegastrointestinaltract.Turnbaughetal.haveproposedtheideaofacoresetoffunctionswithinthemicrobiome,andthetoolsofproteomicsandmetabolomicsmayberequiredformorein-depthfunctionalanalyses
(7,
9).Fromasystemsperspective,metagenomicanalysesmayprovidefurtherdetailsonspecificintraindividualchangesandthushavemajorimplicationsforpersonalizedmedicinestrategies.
Metatranscriptomics,metaproteomics,andmetabonomicswillbeusefultoexplorethefunctionalaspectsofthegutmicrobiomefromthetopdown.Realtimeanalysisoftheintestinalmicrobiomeisausefultoolinthedevelopmentofpersonalizedapproachestotargetedtherapies.Metabonomicscanbedescribedasthestudyofmetabolicresponsestochemicals,theenvironment,anddiseasesandinvolvesthecomputationalanalysisofspectralmetabolicdatathatprovideinformationontemporalchangestospecificmetabolites.Inaddition,metabonomicsprovidesglobalmetabolicprofilingofanindividualinrealtime.Itispossible,withsuchapproaches,toelucidatecomplexpathwaysandnetworksthatarealteredinspecificdiseasestates.Thecombinationofmetabolicprofilingandmetagenomicstudiesofgutmicrobiotapermitsthestudyofhostandmicrobialmetabolismingreatdetail.Suchanalysisoffunctionalcomponentsofthemicrobiomethataffectmetabolismandhumanhealthisreferredtoasfunctionalmetagenomics.
Metagenomicsandthescienceofthehumanmicrobiomehavearrivedattheforefrontofbiologyprimarilybecauseofmajortechnicalandconceptualdevelopments.Themajortechnicaldevelopmentwasthedeploymentinmanycentersofnext-generationDNAsequencingtechnologieswithgreatlyenhancedcapabilitiesforsequencingcollectionsofmicrobialgenomesinthemetagenome.Technologicaladvanceshavecreatednewopportunitiesforthepursuitoflarge-scalesequencingprojectsthatweredifficulttoimagineadecadeago.Thekeyconceptualdevelopmentwastheemergingparadigmoftheessentialnatureofcomplexmicrobialcommunitiesandtheirimportancetomammalianbiologyandhumanhealthanddisease.TheHumanMicrobiomeProjectwasapprovedinMay2007as1of2majorcomponents(inadditiontothehumanepigenomicsprogram)ofNIHRoadMapversion1.5(nowknownastheCommonFund).Recently,2seminalreportsfromtheHumanMicrobiomeProjectconsortium
(10,
11)
describedinvestigationsinwhichapopulationof242healthyadultsweresampledat15or18bodysitesupto3times,5177microbialtaxonomicprofilesweregeneratedfrom16SrRNAgenes,andmorethan3.5Tbasesofmetagenomicsequencesweregenerated.Inaddition,inparallel,theHumanMicrobiomeProjectconsortiumhassequencedapproximately800human-associatedreferencegenomes.Thisresourcewillprovideaframeworkforfuturestudiesofdiseasestatesandareferencecollectionofhealthyhumanmicrobiomedata.Thedatasetwillenablefutureinvestigationsintotheepidemiologyandecologyofthehumanmicrobiomeinvariousdiseasestates,andtreatmentstrategieswillevolvefromthesestudies.Usingcompositionalandfunctionalapproaches,therelationshipsbetweenpathologicalvariationsinthegutmicrobiomeandseveraldiseasestateshavebeendelineated.
Urinemetabolomicsprovidesanopportunityforstudiesofthemicrobiome’simpactonwhole-bodymetabolism.Theadvantagesofusingurinarysamplesincluderelativelylargesamplevolumesandtheconvenienceofnoninvasivecollection.Inaddition,urinesamplescanbeusedfortheinvestigationofthechronologyofmetabolicchangesandthusareavaluabletoolforinvestigationsrelatedtothepathogenesisorprogressionofdiseaseandforscreeninganddiagnosisaswellasprognosticevaluat
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