Different Chinese herbal medicines the toxic components manshuriensis aristolochic acid A content毕.docx
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Different Chinese herbal medicines the toxic components manshuriensis aristolochic acid A content毕.docx
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DifferentChineseherbalmedicinesthetoxiccomponentsmanshuriensisaristolochicacidAcontent毕
DifferentChineseherbalmedicinesthetoxiccomponentsmanshuriensisaristolochicacidAcontent
ComparemanshuriensisandLongDanXieGantrendofaristolochicacidAdemolitionpartygroupofChineseherbalmedicinesonthecontentofaristolochicacidA[method][Abstract]observedproportionofLongdanoriginalsideforthetestsamplepointssevengroups:
GuanMuTonggroup,fullpartygroupmanshuriensis+Qingregroup,manshuriensis+Ziyingroup,GuanMuTong+Leewatergroupmanshuriensis+licoricegroup,negativecontrolgroup(afullgooffwoodpass,RP-HPLCchromatography(RPHPLCmethodtoDiamonsilTM(diamonds)C18(250mm×4.6mm,of5μmcolumnwithmethanol-water-glacialaceticacid(volumeratio74:
24:
1)asthemobilephase,detectionwavelength315nmweremeasuredaristolochicacidAinthegroup.[Results]ComparedwithmanshuriensisgroupLongdanprescriptionlowestcontentofaristolochicacidAmanshuriensis+ZiyingrouparistolochicacidAcontentwassignificantlyreduced.[Conclusion]undertheguidanceofthetheoryofprescriptioncompatibilitythereasonablecompatibilityitisfeasibletoreducethetoxicityofmanshuriensis.
[Keywords]manshuriensischemicalLongdanaristolochicacidAanalysisofChineseherbalmedicineschromatographyhighperformanceliquidTherelatedwoodthepass(ofCaulisAristolochiaeManshuriensis,CAMtheAristolochicofAristolochiamanshruiensisplantnortheastofaristolochicKom.Driedcane,itsmainchemicalcomponentsofaristolochicacidA,B,D,thearistolochicDether,Magnoliaalkalioleanolicacid[1]thebittercoldtoxicmanshuriensis,pureheartandfire,Leeurine,passthroughtheeffectofmilkformouthsores,upsetredurine,edema,hotshowerastringentpain,vaginaldischarge,amenorrhealessmilk,hotandhumidTongbietc.Modernresearchshowsthatitspharmacologicaleffectsdiuretic,anti-mutagenic,anti-tumorandbloodpressureloweringeffect[2].
Manshuriensiscontainaristolochicaciddamagetohumankidneys,theStateFoodandDrugAdministrationissuedanoticeinJune2003tocancelitsmedicinalstandardThismeansmanshuriensis,willneverbeabletouseasalegitimateformoftraditionalChinesemedicine.AsuccessiveapplicationmostfamoussquareLongdan,(LongdanXieganDecoction,LXD,forexample,toobservewhetherChineseherbalmedicinescanreducemanshuriensistoxicitytoverifytheprescriptioncompositiontheoryofrationality.
Variorum>>From<<LongDanXieGanmedicalside,diarrheathehepatobiliaryrealfire,clearedunderthenameoffocushumidsquareontheinflammationtheattendinghepatobiliaryrealfire,liverandgallbladderdampbetcard.Inrecentyears,athomeandabroadcontinuetohavereportedtakingLongdanxieganleadtokidneydamage,andmoreirrationaluseoflong-termover-taking[3-5]LongDanXieGanapplicationsforhundredsofyearsbutithasnotadversereactionstotheexperimentbyreversed-phasehighperformanceliquidchromatography(RPHPLC)methodobservecontentLongdanitsdemolitionpartygrouponmanshuriensisaristolochicacidA(adritolochicacid,AA),toexplorethetraditionalChinesemedicinecompatibilityreducemanshuriensistoxicityoflaw.
1instrumentandreagent1.1InstrumentDionexSummitP680highperformanceliquidchromatograph(AmericanDIONEX)theChromeleonchromatographyworkstation(USADIONEX,UVD170UUVdetector(theUnitedStatesDIONEXcompany.
1.2DrugsReferencesubstancearistolochicacidAisprovidedbytheNationalDrugandBiologicalProducts(batchnumber:
110746200406gentian,skullcap,gardenia,Alisma,angelica,rehmannia,Plantago,Bupleurum,rawlicoriceScrophulariaceae,OphiopogonpurchasedfrompharmaciesFirstAffiliatedHospitalofGuangzhouUniversityofTraditionalChineseMedicine,GuanMuTongispurchasedinHebeianguomedicinemarket,TCMEvaluationDepartmentoftheGuangzhouUniversityofTraditionalChineseMedicineProfessorLiWeiidentificationofAristolochiamanshruiensisKom..
1.3ReagentsMethanolHPLCgrade,glacialaceticacid,anhydrousmethanol,hydrochloricacidandetherwereofanalyticalgrade,waterpurewaterC’estbon.
2MethodsandResults2.1ChromatographicconditionsColumn:
DiamonsilTM(diamonds)C18(250mm×4.6mm,5μmdetector:
UVD170UUVdetection,detectionwavelength:
315nm,columntemperature:
roomtemperature(25°C),mobilephase:
methanol-water-aceticacid(volumeratio75:
24:
1;theflowrate1mL/min,injectionvolume5μL.
2.2PreparationofsamplesUnderthemedicalsideVariorum>>Note:
“thegentianofdiarrheaJueyinofhot,theBupleurumflatShaoyangheat,skullcap,thegardeniaofQingfeiwithsanjiaoheattorankandfileofAlismadiarrheakidneythroughthewetwoodpass,thePlantagodiarrheabladderwetrankandfileofthedownwardspiralareasbittercolddrug,sobynormalization,andnourishingandBugan,thelicoricetoslowwithoutinjuryinthestomach,sotheminister“willFangzhongLonggallgrass,Bupleurum,Scutellaria,Gardeniaisclassifiedasantipyretic,willAlisma,PlantagonormalizedordertofacilitatewatermedicineAngelica,habitatclassifiedasYinanddrugdosesproportionoftheoriginalsquare,dividedintosevengroups:
manshuriensisgroup:
OffAkebia3g,all-partygroup:
OffAkebia3g,gentian2g,theskullcap3g,thegardenia3g,Alisma4g,theAngelica1g,Rehmanniae3gPlantago3gBupleurum2g,rawlicorice2g,manshuriensis+QingreGroup:
GuanMuTong3g,2ggentian,yellowbaicalensis3g,gardenia3g,Bupleurum2g,manshuriensis+Ziyingroup:
GuanMuTong3g,Angelica1g,Rehmanniae3g,manshuriensis+Leewatergroup:
GuanMuTong3g,Alisma4g,carformersub-3g,manshuriensis+licoricegroup:
OffAkebia3g,rawlicorice2g,negativecontrolgroup:
gentian2g,theskullcap3g,thegardenia3g,Alisma4g,Angelica1g,therehmannia3g,thePlantago3g,Bupleurum2g,therawlicorice2g.
Forthetestsample,addingwater400mL,soaking30min,boiling60min,andfilteredwithgauze.Dregspluswater100mlboiling30mingauzefilter.Merge2timesthefiltratewasconcentratedto50mL,thelessons10mL,with6mol/LhydrochloricpH=2,transferredtoaseparatoryfunnelandextractedwithetherfourtimesby[6](10mL,5ml×3,extractswerecombined,waterbathandevaporatedtodryness,dissolvedinmethanol,andconstantvolumeina10mLvolumetricflask.through0.45μmofmicroporousmembranefiltration,thatis,too.
2.3PreparationofthereferencesolutionAccuratelyweighedaristolochicacidAreferencesubstance,placedina10mLvolumetricflask,dissolvedinmethanol,anddilutetothemark,shake,thepreciseamountof1mLhome10mLvolumetricflask,dissolvedinmethanol,anddilutetovolume,theshakemade0.034mg/mLsolutionthatwas.
2.4linearrelationshipbetweenInaccordancewiththeabovechromatographicconditions,accuratelyweighedaristolochicacidAreferencesolution2.5,5,10,15,20μLpeakareaabscissaXinjectionvolumeordinateYstandardcurveresultshorseAristolochicacidlinearrangeof0.084~0.680μg,equationY=0.0446X-0.0173,R=0.9998(Figure1.
2.5precisiontestTakearistolochicacidAstandardsolutionconsecutiveinjectionsof5times,measuredaristolochicacidApeakareasR1.8%.
2.6StabilityTestTheresultsshowedthataristolochicacidAgoodstabilitywithin6h0,2,4,6hinjection4timespasssamplesunderthesameconditions,themeasuredpeakarea.AristolochicacidAcontentsR2.6%.Fetchtherelevantwood.
2.7RepeatabilitytestSameof1partmanshuriensissamples,samplemeasurementmethodsamebelowoperation,parallelmeasuredthreetimestocalculatethecontentofaristolochicacidA1.7%sR.
2.8TheaveragerecoveryexperimentPrecisionWeightakesamplesofknowncontentofthesample,respectively,addingacertainamountofaristolochicacidA2.2operationwiththedetermination,andtheamountbasedonthecontentofthesampleandthereferencesubstanceofaristolochicacidAcalculationofrecovery.anaveragerecoveryof99.3%ofthesampledeterminationLinkstofreepapersDownloadCenter
2.9
Ofeachgroupthesamplesolution,2.1ChromatographicconditionsweremeasuredandtheresultsareshowninTable1,Figure2.
Table1show,Longdandifferentcompatibilities,manshuriensisaristolochicacidAcontentcomparisonareasfollows:
afullgroup<manshuriensis+Ziyingroup<OffAkebia+Leewatergroup<manshuriensis+Qingregroup<offwoodthroughgroup<manshuriensis+licoricegroupshowthattheall-partygroupofaristolochicacidAdecreasedup.Table1LongdananddemolitionpartygroupofaristolochicacidAcontent
3DiscussionLongdanclinicalusehasbeenhundredsofyearsofhistory.Ancientmedicalpracticeoflong-term,mastercompatibilityrulemanshuriensis.,UndertheguidanceoftheChinesemedicinetheory,instrictaccordancewiththeLongdanindications,dosageandprecautions,rationaluse,youcanreduceoravoidtheoccurrenceofsideeffects[7].manshuriensisrenaltoxicityfromthetraditionalChinesemedicinetheoryintermsofbelongingdiuresisShangyinLongdancompatibilityZiyindrugs(habitat,AngelicatoraiseBuganbloodtotopreventdruginfiltrationLeeShangyin.manshuriensisZiyindrugscombinedtheresultsofthisexperimentcansignificantlyreducethecontentofaristolochicacidA,inlinewiththetraditionalChinesemedicinetheoryofcompatibility,undertheguidanceofTCMtheoryprescriptioncompatibilityreasonable.reportedintheliterature[8],thetreatmentresultsmanshuriensispoisoningcases,evenservedChinesemedicineLiuWeiDiHuangPilldisabledialysisinstablecond
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