Biorad C1000ManagerPCR说明书.docx
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Biorad C1000ManagerPCR说明书.docx
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BioradC1000ManagerPCR说明书
Copyright©2007Bio-RadLaboratories,Inc.Reproductioninanyform,eitherprintorelectronic,isprohibitedwithoutwrittenpermissionofBio-RadLaboratories,Inc.
Chill-Out,iProof,iTaq,andthehelixlogoaretrademarksbelongingtoBio-RadLaboratories,Inc.
Otherbrandsorproductnamesaretrademarksoftheirrespectiveholders.
LICENSENOTICETOPURCHASER
Purchaseofthisinstrument,SerialNo.____________,conveysalimitednon-transferableimmunityfromsuitforthepurchaser’sowninternalresearchanddevelopmentandforuseinappliedfieldsotherthanHumanInVitroDiagnosticsunderoneormoreofU.S.PatentsNos.5,656,493,5,333,675,5,475,610(claims1,44,158,160-163and167only,and6,703,236(claims1-7only,orcorrespondingclaimsintheirnon-U.S.counterparts,ownedbyAppleraCorporation.Norightisconveyedexpressly,byimplicationorbyestoppelunderanyotherpatentclaim,suchasclaimstoapparatus,reagents,kits,ormethodssuchas5’nucleasemethods.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.
S1000ThermalCyclerManual|
SafetyandRegulatoryCompliance
TheS1000™thermalcyclerheatsandcoolsquicklyduringoperation.
Westronglyrecommendthatyoufollowthesafetyspecificationslisted
inthissectionandthroughoutthismanual.
SafetyWarningLabels
Warninglabelspostedontheinstrumentandinthismanualwarnyou
aboutsourcesofinjuryorharm.RefertoTable1toreviewthemeaning
ofeachsafetywarninglabel:
InstrumentSafetyWarnings
Thefollowingwarningslabelsdisplayontheinstrument,andrefer
directlytothesafeuseofthisS1000thermalcycler:
Table1:
Meaningofsafetywarninglabel.
CAUTION:
Riskofdanger!
Thissymbolidentifiescomponentsthatposeariskof
personalinjuryordamagetotheinstrumentifimproperlyhandled.Whereverthissymbolappears,consultthemanualforfurtherinformationbeforeproceeding.CAUTION:
Riskofelectricalshock!
Thissymbolidentifiescomponentsthatposeariskofelectricalshockifimproperlyhandled.
CAUTION:
Hotsurface!
Thissymbolidentifiescomponentsthatposeariskofpersonalinjuryduetoexcessiveheatifimproperlyhandled.
Table2:
Instrumentsafetywarninglabels.
Icon
MeaningWarningaboutriskofharmtobodyorequipment.
OperatingtheS1000thermalcyclerinstrumentbeforereadingthismanualcanconstituteapersonalinjuryhazard.Onlyqualifiedlaboratorypersonnelshouldoperatethisinstrument.
Warningaboutriskofharmtobodyorequipmentfromelectricalshock.
Donotattempttorepairorremovetheoutercaseofthisthermalcyclerbase,powersupply,heatpump,orotheraccessories.Ifyouopentheseinstrumentsyouput
yourselfatriskforelectricalshockandvoidyourwarranty.Allrepairsmustbedonebyanauthorizedrepairservice.
Neverremovetheoutercaseofathermalcyclerbasebecauseyoumightreceiveanelectricalshock.
Thisthermalcyclerusesneutralfusing,whichmeansthatlivepowercouldstillbeexposedinsidetheinstrumentevenwhenthefuseisblownorremoved.
Warningaboutriskofburning.
Athermalcyclergeneratesenoughheattocauseseriousburns.Wearsafetygogglesorothereyeprotectionatalltimesduringoperation.Alwaysallowthesampleblocktoreturntoidletemperatebeforeopeningthelidandremovingsamples.Alwaysallowmaximumclearancetoavoidaccidentalskinburns.
SafetyUseSpecificationsandCompliance
Thisinstrumenthasbeentestedandfoundtobeincompliancewithallapplicablerequirementsofthefollowingsafetyandelectromagneticstandards:
SAFETYCOMPLIANCE
Thisinstrumenthasbeentestedandfoundtobeincompliancewithallapplicablerequirementsofthefollowingsafetyandelectromagneticstandards:
•ULStdNo.61010A-1ElectricalEquipmentforMeasurement,Control,andLaboratoryUse,Part1:
GeneralRequirements
•ULStdNo.61010A-2-010ElectricalEquipmentforMeasurement,Control,andLaboratoryUse,Part1:
GeneralRequirements
•CAN/CSAC22.2No.1010.1-92-SafetyRequirementsforElectrical
EquipmentforMeasurement,Control,andLaboratoryUse,Part1:
GeneralRequirements(includesAmendment1
•CAN/CSAC22.2No.1010.1B-97-Amendment2CAN/CSAC22.2No.1010.1-92-SafetyRequirementsforElectricalEquipmentforMeasurement,
Control,andLaboratoryUse,Part1:
GeneralRequirements
•CAN/CSAC22.2No.1010.2.010A-97-SafetyRequirementsforElectricalEquipmentforMeasurement,Control,andLaboratoryUse,Part2-010:
ParticularRequirementsforLaboratoryEquipmentfortheHeatingof
Materials,AmendmentNo.1
•EC61010-1SafetyRequirementsforElectricalEquipmentforMeasurement,Control,andLaboratoryUse,Part2-010:
ParticularRequirementsfor
LaboratoryEquipmentfortheHeatingofMaterials
ELECTROMAGNETICCOMPATIBILITY(EMC
•F.C.CTitle47Part15BasaClassAdigitaldevice.Warningaboutriskofexplosion.
Thesampleblockscanbecomehotenoughduringthecourseofnormaloperationtocauseliquidstoboilandexplode.
Table2:
Instrumentsafetywarninglabels.
IconMeaning
Table3:
SafeusespecificationsSafeuserequirements
SpecificationsInputpower
Rated100–240VAC,50–60Hz.Fuses
250V,10A.Temperature
IndooruseAmbienttemperatureof5oCto31oC.Relativehumiditymaximumof80%noncondensing.Altitude
Upto2,000metersabovesealevel.Overvoltage
Categories
IIPollutiondegree2
•EN61326ClassAElectricalEquipmentformeasurement,control,andlaboratoryuse-EMCRequirements.
FCCWARNINGSANDNOTES
•Warning:
Changesormodificationstothisunit,notexpresslyapprovedbythepartyresponsibleforcompliance,couldvoidtheuser’sauthoritytooperatetheequipment.
•Note:
ThisequipmenthasbeentestedandfoundtocomplywiththelimitsforaClassAdigitaldevice,pursuanttopart15oftheFCCRules.Theselimitsare
designedtoprovidereasonableprotectionagainstharmfulinterferencewhentheequipmentisoperatedinacommercialenvironment.Thisequipment
generates,uses,andcanradiateradiofrequencyenergyand,ifnotinstalledandusedinaccordancewiththeinstructionmanual,maycauseharmfulinterferencetoradiocommunications.Operationofthisequipmentinaresidentialareaislikelytocauseharmfulinterferenceinwhichcasetheuserwillberequiredtocorrecttheinterference,athisownexpense.
•NoteregardingFCCcompliance:
AlthoughthisdesignofinstrumenthasbeentestedandfoundtocomplywithPart15,SubpartBoftheFCCRulesforaClass
Adigitaldevice,pleasenotethatthiscomplianceisvoluntary,fortheinstrument
qualifiesasan“exempteddevice”under47CFR15.103(c,inregardtothecitedFCCregulationsineffectatthetimeofmanufacture.
•NoteregardingCanadianEMCcompliance:
Lepresentappareilnumeriquen’emetpasdebruitsradioelectriquedepassantleslimitesapplicablesaux
appareilsnumeriquesdeclassAprescritesdanslereglementsurlebrouillageradioelectriqueedicteparleMinisteredesCommunicationsduCanada.
•Cables:
ShieldedcablesmustbeusedwiththisunittoensurecompliancewiththeClassAFCClimits.
•UseonlyBio-RadUSBcable(CatalogNo.184-8000withyour1000Seriescyclers.
S1000ThermalCyclerManual|SafetyandRegulatoryCompliance
TableofContents
Chapter1.Introduction.........................................1TheBio-Rad1000SeriesPlatform..................................1
S1000ThermalCyclerPerformanceandFeatures....................2
Bio-RadResourcesandReferences................................3
WritingConventionsUsedinThisManual...........................4Chapter2.GetStarted..........................................5SettingUptheS1000ThermalCycler...............................5
ThermalCyclerOverview.........................................7FrontViewwithoutReactionModule7
TopViewwithReactionModule7
BackViewwithReactionModule8
InterchangeableReactionModules.................................9InsertingandRemovingaReactionModule10
OpeningandClosingtheAdjustableLid............................12OpeningtheLid12
ClosingtheLidandSettingLidForce13
ControlPanelKeysandMainMenu...............................13ControlPanelKeys15
TheMainMenu16
TheFileTree..................................................16Chapter3.CreateandEditProtocols.............................19CreatingaProtocolFilewiththeNEWOption........................19
AddingINC,EXT,RATE,andBEEPParameters........................28AddinganIncrement(INCtoaTemperatureStep28
Extending(EXTtheHoldTimeinaTemperatureStep30
ChangingtheRampRate(RATEinaTemperatureStep31
AddingaBeep(BEEPtoaTemperatureStep32
SettingTemperatureControlMode................................33
ChoosingLidTemperature.......................................33
ChanginganExistingProtocolwiththeEDITOption..................34Chapter4.RunExistingProtocols...............................41RunningaProtoc
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