1、introduction写作方法及技巧introduction写作方法及技巧科技学术论文Introduction引言的主要内容是交代此项研究的来龙去脉，简要说明课题的缘起与背景，性质与意义，动机与目的、主要理论根据及其基本原理等，同时指出相关领域内前任的研究成果、存在问题和知识空白，以表明本项研究的连续性和需要性，叙述有关本课题的历史沿革是为了温故而知新，但应注意掌握适当的范围和尺度，一般来说仅需要介绍极密切的有关史料即可，不宜泛泛赘述大量历史文献，否则会造成Introduction长而乏味。first：提出研究现状和此研究的重要性second：强调有必要解决存在的问题third：介绍作者自己
2、的研究内容、提出创新性逻辑的连贯内容的创新词汇简洁时态1. What is an introduction?The introduction section shows the questions that should be answered for the readers once they finish reading the “Introduction”. 2. Whats the purpose of the introduction?The introduction comes at the start of a piece of writing. Without this par
3、t, the reader cannot easily understand the more detailed information about the research that comes later in the thesis. It introduces:（1）.the research by situating it (by giving background), （2）.presenting the research problem , and saying how and why this problem will be solved ,(9) gives details a
4、bout the methodology详细描述了论文中所用的方法(10) announces the findings公布了论文的结论6. Four components of a model.（1）Establish the importance of your fieldProvide background/ facts/information (possibly from research) Define the terminology in the title/key words Present the problem area/current research focus 确立研究
5、领域的重要性提供背景事实或信息（有可能来自现有文献）定义题目或关键词中的术语给出所研究问题的范畴或目前的研究重点（2）Previous and/or current research and contributions 前期的研究或目前的研究及其贡献（3）Locate a gap in the research Describe the problem you will address Present a prediction to be tested 确定已有研究工作的空白；描述你要解决的问题呈现要验证的预测（4）Describe the present paper 描述现在的论文 7.Gr
6、ammar and writing skills.语法 时态 写作技巧8. Vocabulary词汇的简洁举例三篇文章：1.Gene expression profiling and pathway analysis of hepatotoxicity induced by triptolide in Wistar rats在Wistar大鼠中，通过基因表达谱和通路分析由雷公藤甲素诱导的肝毒性引言的主要内容是交代此项研究的来龙去脉，例如本文中，简要说明课题的缘起与背景， TP的性质Triptolide (diterpenoid triepoxide, TP), purified from th
7、e shrublike vine Tripterygium wilfondii Hook F (TWHF)与药理学意义possesses anti-inflammatory, anti-fertility, anti-neoplastic and immunosuppressive activities 实验的动机However, clinical use of TWHF or TP has been limited due to severe adverse reactions, such as gastrointestinal upset, diarrhea, reproductive t
8、oxicity and problems associated with circulatory systems目的we hypothesized that liver is a major toxic target of TP treatment. Thus, it is essential to elucidate the mechanism of TP-induced hepatotoxicity from a safety point of view.the aim of our study was to identify candidate genes associated with
9、 TP treatment and to provide novel insights to better elucidate the mechanisms of toxic effects of TP.主要理论根据及其基本原理Considering that microarray technology is recognized as a reliable toxicologica method to determine mechanisms of drug-induced toxicity, identify biomarkers and to predict chemical toxic
10、ity.Therefore, the aim of our study was to identify candidate genes associated with TP treatment and to provide novel insights to better elucidate the mechanisms of toxic effects of TP.同时指出相关领域内前任的研究成果possesses anti-inflammatory, anti-fertility, anti-neoplastic and immunosuppressive activities (Chen
11、, 2001; Huynh et al., 2000; Panichakul et al., 2006). have emerged as treatments of rheumatoid arthritis, systemic lupus erythematosus, nephritis, leprosy and asthma (Lipsky and Tao, 1997; Liu et al., 2005; Zhang et al., 2010). clinical use of TWHF or TP has been limited due to severe adverse reacti
12、ons, such as gastrointestinal upset, diarrhea, reproductive toxicity and problems associated with circulatory systems (Hikim et al., 2000; Ni et al., 2008; Yang et al., 2012; Zhang et al., 2011).Recently, hepatotoxicity induced by various extracts of TWHF in animals and humans has been reported by m
13、any researches (He et al., 2006; Mei et al., 2005; Wang et al., 2007)To date, only mitochondrial respiratory chain inhibition, lipid peroxidation, DNA damage and hepatocyte apoptosis were proposed to be involved in TP-induced liver injury (Fu et al., 2011; Mei et al., 2005; Yao et al., 2008).等存在问题和知
14、识空白 Hepatic differential gene expression was analyzed using oligonucleotide microarray analysis for over-represented functions and phenotypically anchored to complementary histopathologic, biochemical, and dosimetry data in the liver. The results indicate that TP affects diverse cellular pathways, i
15、ncluding insulin signaling pathway, glucose metabolism, cell cycling, oxidative stress and apoptosis. These data provide a clearer understanding of the molecular mechanisms of TP-induced hepatotoxicity, as well as useful information for predicting drug hepatotoxicity.以表明本项研究的连续性和需要性，叙述有关本课题的历史沿革是为了温
16、故而知新，Triptolide (diterpenoid triepoxide, TP), purified from the shrublike vine Tripterygium wilfondii Hook F (TWHF), possesses anti-inflammatory, anti-fertility, anti-neoplastic and immunosuppressive activities (Chen, 2001; Huynh et al., 2000; Panichakul et al., 2006). Recently, the methanol/chlorof
17、orm (T2) and ethyl acetate (EA) extracts of TWHF, in which TP was identified as the principal active compound, have emerged as treatments of rheumatoid arthritis, systemic lupus erythematosus, nephritis, leprosy and asthma (Lipsky and Tao, 1997; Liu et al., 2005; Zhang et al., 2010). However, clinic
18、al use of TWHF or TP has been limited due to severe adverse reactions, such as gastrointestinal upset, diarrhea, reproductive toxicity and problems associated with circulatory systems (Hikim et al., 2000; Ni et al., 2008; Yang et al., 2012; Zhang et al., 2011).Recently, hepatotoxicity induced by var
19、ious extracts of TWHF in animals and humans has been reported by many researches (He et al., 2006; Mei et al., 2005; Wang et al., 2007). Besides, Liu et al., (2010) found that potential hepatotoxicity in rats treated with TP for 28 days was associated with increasing levels of serum alanine aminotra
20、nsferase (ALT) and aspartate aminotransferase (AST) (Liu et al., 2010). Moreover, it was reported that oral administration of TP to rats could lead to liver injury or even death (Fu et al., 2011). In addition to this, our previous investigation showed that the concentration of TP found in liver exce
21、eds those observed in other tissues, such as spleen, lung, heart, and kidney (unpublished data). On account of this, we hypothesized that liver is a major toxic target of TP treatment. Thus, it is essential to elucidate the mechanism of TP-induced hepatotoxicity from a safety point of view.Unfortuna
22、tely, its underling mechanisms are still insufficiently recognized. To date, only mitochondrial respiratory chain inhibition, lipid peroxidation, DNA damage and hepatocyte apoptosis were proposed to be involved in TP-induced liver injury (Fu et al., 2011; Mei et al., 2005; Yao et al., 2008). Conside
23、ring that microarray technology is recognized as a reliable toxicologica method to determine mechanisms of drug-induced toxicity, identify biomarkers and to predict chemical toxicity (Lee et al., 2010; Wang et al., 2011). Therefore, the aim of our study was to identify candidate genes associated wit
24、h TP treatment and to provide novel insights to better elucidate the mechanisms of toxic effects of TP.Here, we describe genome-wide gene expression in the TP-exposed Wistar female rat liver. Differential gene expression was evaluated in 6-week-old female Wistar rat livers following 14 days of conti
25、nuous exposure to large doses of TP. Hepatic differential gene expression was analyzed using oligonucleotide microarray analysis for over-represented functions and phenotypically anchored to complementary histopathologic, biochemical, and dosimetry data in the liver. The results indicate that TP aff
26、ects diverse cellular pathways, including insulin signaling pathway, glucose metabolism, cell cycling, oxidative stress and apoptosis. These data provide a clearer understanding of the molecular mechanisms of TP-induced hepatotoxicity, as well as useful information for predicting drug hepatotoxicity
27、.2.综述Blood vessels, a potential therapeutic target in rheumatoid arthritis?血管，类风湿性关节炎潜在的治疗靶标？IntroductionRheumatoid arthritis (RA) can be defined as a disease of the blood vessels, both micro- and macro-vessels. The formation of new micro-vessels is in fact necessary to afford the nutritional supply
28、 to proliferating synovial pannus, while macro-vessels are the site where accelerated atherosclerosis driven by diseases systemic inflammation develops. New vessels formation on one side, and atherosclerotic plaque progression on the other, might seem two different biological phenomena, the first re
29、lated to the articular involvement of the disease, the second to its main systemic complication. In this context, targeting blood vessels in RA might mean either attempting to reduce synovial vascular supply starving the synovial pannus limiting its proliferation or, in the other case, trying to lim
30、it macro-vessels damage outside the joint. In this review we will analyse the possibility of targeting synovial microvessels to treat rheumatoid arthritis, but we will discuss as well the evidence supporting a link between micro- and macro-vascular involvements in RA.综述的介绍，介绍所提到物质的基本概念，简要说明课题的缘起与背景，
31、RA与血管生成相关，与血管生成的必要性，在这里，说明该文章立题的主要依据与主要原理，并提出在此综述中接下来会说到的内容，如：作者将分析滑膜微血管治疗类风湿关节炎的可能性，且讨论，血管与RA微观和宏观之间联系的证据。概念由浅入深，词汇简介，介绍基本概念时使用一般时态，提到文章后续会介绍的内容时使用将来时，对未来内容的展望。3.介绍功能性文章Dissection of TNF Receptor 1 Effector Functions: JNK Activation Is Not Linked to Apoptosis While NF-kB Activation Prevents Cell De
32、ath肿瘤坏死因子受体1的效应功能：NF-kB的活化阻止细胞死亡，JNK活化未关联凋亡这篇文章的简介有1318词，系统的介绍了TNF的含义，分类，受体类型，相关的两个转录因子，AP-1、 NF-kB 、TNF所经过的通路，通路中所涉及的许多其他细胞因子，由于是介绍功能性文章，这样的背景知识很重要，所以虽然Introduction很长，但是有意义。逻辑连贯由各部分功能连接到总体功能，均采用一般时介绍，时态统一。Tumor necrosis factor (TNF) is a cytokine produced by many cell types, including macrophages, monocytes, lymphoid cells, and fibroblasts, in response to inflamma-tion, infection, and other environmental challenges (Tra-cey and Cerami, 1993). TNF elicits a wide spectru of organismal and cellular responses,
