1、生化检测方法汇总生化检测方法汇总laparici de tot tipus de casos, la caiguda daccident de sha produt. (9) on la feina nivells sense operacions de installacions de protecci de seguretat, treballadors de la construcci han de dur un cintur o arnesos de seguretat (dispositius de protecci de seguretat sha comprovat abans
2、del seu s), el cintur de seguretat o cordes de seguretat utilitzats han de complir els principis dalta i baixa. No hi ha deures don ls de productes de protecci, per evitar accidents de caiguda passi. 6, prevenci dintoxicacions, asfxia accident les mesures preventives per evitar lenverinament, millor
3、 protecci de la seguretat i la salut dels treballadors, les mesures es formulen. (1) quan la profunditat de la pila s ms de 5 M, el dia abans de linici de ls detector de gas per a la detecci de gasos txics, confirmar que el gas dins del forat correctament, abans dobra menor; ha de tenir 15min noms d
4、esprs de la voladura de fum forat es permeten les operacions. (2) lalberg est estrictament prohibit en lemmagatzematge de substncies txiques i qumics. (3) s interior lestabliment independents Calefacci, no fan de flama oberta denllumenat, Calefacci, per evitar accidents que requereixen denverinament
5、 de monxid de carboni independents de lhabitatge Cambra climatitzada amb foc de carb, estufes ha de ser situats a lexterior. (4) al menjador de lautoritzaci de sanejament, personal de cuina ha de tenir un certificat de salut i certificat mdic per treballar. Botiga daliments crus i cuinats ha de ser
6、processat per separat. for safety and security, set up security groups, head of the work group is responsible for the operations of safety and security, each team full-time safety officer is responsible for the daily life and production security. 成 . Good dining, on the implementation of special det
7、ection system for procurement of food, preventing the purchase of spoilage and poisonous food to prevent food poisoning accident occurred. Pay attention to personal and environmental hygiene, to beautify the environment, clean air, beautiful life, environment, prevent the spread of infectious diseas
8、e epidemics and ensure attendance to ensure production safety. 8.4.4.4 from management to ensure safety punishment detailed rules for the implementation of a safety. To comply with policies, laws and regulations, observe labour discipline, according to safety regulations and technical specifications
9、 for construction requirements for the construction, maintenance of production and life order, maintain social order there is significant merit in team and individual Award respectively; otherwise, penalties, to ensure production safety. 8.4.4.5 construction site safety protection 1) temporary facil
10、ities security protection a. construction of establishing traffic signs and lighting, vehicle and pedestrian traffic safety. B. underground structure within the construction site, the underground pipeline,一、血清谷丙转氨酶(SGPT)赖氏改良法 器材:试管及试管架、移液器或吸量管、恒温水浴锅、721型分光光度计、温度计、动物血清 试剂: (1)0.1mol/L pH7.4磷酸盐缓冲液: ?0
11、.1mol/L磷酸氢二钠溶液:称取Na2HPO414.22g或Na2HPO4?2H2O17.8g溶 解于蒸馏水中，并稀释至1 000ml,4?保存。 ?0.1mol/L磷酸二氢钾溶液:称KH2PO413.61g溶解dH2O中，稀释至1000ml, 4?保存。 ?将0.1mol/L磷酸氢二钠溶液420ml和0.1mol/L磷酸二氢钾溶液80ml混和即 为0.1mol/L pH7.4的磷酸盐缓冲液。加氯仿数滴，4?保存。 (2)标准丙酮酸(含丙酮酸2.0mol/mL) : 取标准丙酮酸钠10mg，用上述缓冲液准确定容为50mL。此液须当日用当日配。 (3)SGPT底物液(含-酮戊二酸2mol/ml
12、及DL-丙氨酸200mol/ml) : 取-酮戊二酸29.2mg，DL-丙氨酸7g ,用试剂1溶成100ml，在稀释到刻度前，以1mol /L NaOH调pH 7.4(因为转氨酶在pH7.3以下或7.6以上的环境中酶活性下降)，加数滴氯仿防腐。此液于冰箱保存可使用4天。 (4)GOT底物液(DL天冬氨酸200mmol/L ,-酮戊二酸 2mmol/L):称取- 酮戊二酸29.2mg和DL-门冬氨酸2.66g置于一小烧杯中,加入1mol/L氢氧化钠20.5ml使溶解,并校正pH至7.4,然后将溶液移入100ml容量瓶内,用0.1mol/L磷酸盐缓冲液定容至刻度。放置冰箱内保存。 (5)2.4-二
13、硝基苯肼溶液: 取分析纯2,4-二硝基苯肼19.8mg，用1mol/L HCl溶于100ml，置棕色瓶中保存。 (6)0.4mol/L NaOH溶液: 称取16g固体NaOH，用蒸馏水溶解，冷却至室温，定容至1000mL，备用。 操作步骤: 1、标准曲线绘制:取试管18支按下表操作。 试 管 号 对 照 1 2 3 4 5 丙酮酸标准液(1毫升2微克分子) 0 0.05 0.10 0.15 0.20 0.25 SGPT或SGOT底物 0.50 0.45 0.40 0.35 0.30 0.25 laparici de tot tipus de casos, la caiguda dacciden
14、t de sha produt. (9) on la feina nivells sense operacions de installacions de protecci de seguretat, treballadors de la construcci han de dur un cintur o arnesos de seguretat (dispositius de protecci de seguretat sha comprovat abans del seu s), el cintur de seguretat o cordes de seguretat utilitzats
15、 han de complir els principis dalta i baixa. No hi ha deures don ls de productes de protecci, per evitar accidents de caiguda passi. 6, prevenci dintoxicacions, asfxia accident les mesures preventives per evitar lenverinament, millor protecci de la seguretat i la salut dels treballadors, les mesures
16、 es formulen. (1) quan la profunditat de la pila s ms de 5 M, el dia abans de linici de ls detector de gas per a la detecci de gasos txics, confirmar que el gas dins del forat correctament, abans dobra menor; ha de tenir 15min noms desprs de la voladura de fum forat es permeten les operacions. (2) l
17、alberg est estrictament prohibit en lemmagatzematge de substncies txiques i qumics. (3) s interior lestabliment independents Calefacci, no fan de flama oberta denllumenat, Calefacci, per evitar accidents que requereixen denverinament de monxid de carboni independents de lhabitatge Cambra climatitzad
18、a amb foc de carb, estufes ha de ser situats a lexterior. (4) al menjador de lautoritzaci de sanejament, personal de cuina ha de tenir un certificat de salut i certificat mdic per treballar. Botiga daliments crus i cuinats ha de ser processat per separat. for safety and security, set up security gro
19、ups, head of the work group is responsible for the operations of safety and security, each team full-time safety officer is responsible for the daily life and production security. 成 . Good dining, on the implementation of special detection system for procurement of food, preventing the purchase of s
20、poilage and poisonous food to prevent food poisoning accident occurred. Pay attention to personal and environmental hygiene, to beautify the environment, clean air, beautiful life, environment, prevent the spread of infectious disease epidemics and ensure attendance to ensure production safety. 8.4.
21、4.4 from management to ensure safety punishment detailed rules for the implementation of a safety. To comply with policies, laws and regulations, observe labour discipline, according to safety regulations and technical specifications for construction requirements for the construction, maintenance of
22、 production and life order, maintain social order there is significant merit in team and individual Award respectively; otherwise, penalties, to ensure production safety. 8.4.4.5 construction site safety protection 1) temporary facilities security protection a. construction of establishing traffic s
23、igns and lighting, vehicle and pedestrian traffic safety. B. underground structure within the construction site, the underground pipeline,laparici de tot tipus de casos, la caiguda daccident de sha produt. (9) on la feina nivells sense operacions de installacions de protecci de seguretat, treballado
24、rs de la construcci han de dur un cintur o arnesos de seguretat (dispositius de protecci de seguretat sha comprovat abans del seu s), el cintur de seguretat o cordes de seguretat utilitzats han de complir els principis dalta i baixa. No hi ha deures don ls de productes de protecci, per evitar accide
25、nts de caiguda passi. 6, prevenci dintoxicacions, asfxia accident les mesures preventives per evitar lenverinament, millor protecci de la seguretat i la salut dels treballadors, les mesures es formulen. (1) quan la profunditat de la pila s ms de 5 M, el dia abans de linici de ls detector de gas per
26、a la detecci de gasos txics, confirmar que el gas dins del forat correctament, abans dobra menor; ha de tenir 15min noms desprs de la voladura de fum forat es permeten les operacions. (2) lalberg est estrictament prohibit en lemmagatzematge de substncies txiques i qumics. (3) s interior lestabliment
27、 independents Calefacci, no fan de flama oberta denllumenat, Calefacci, per evitar accidents que requereixen denverinament de monxid de carboni independents de lhabitatge Cambra climatitzada amb foc de carb, estufes ha de ser situats a lexterior. (4) al menjador de lautoritzaci de sanejament, person
28、al de cuina ha de tenir un certificat de salut i certificat mdic per treballar. Botiga daliments crus i cuinats ha de ser processat per separat. for safety and security, set up security groups, head of the work group is responsible for the operations of safety and security, each team full-time safet
29、y officer is responsible for the daily life and production security. 成 . Good dining, on the implementation of special detection system for procurement of food, preventing the purchase of spoilage and poisonous food to prevent food poisoning accident occurred. Pay attention to personal and environme
30、ntal hygiene, to beautify the environment, clean air, beautiful life, environment, prevent the spread of infectious disease epidemics and ensure attendance to ensure production safety. 8.4.4.4 from management to ensure safety punishment detailed rules for the implementation of a safety. To comply wi
31、th policies, laws and regulations, observe labour discipline, according to safety regulations and technical specifications for construction requirements for the construction, maintenance of production and life order, maintain social order there is significant merit in team and individual Award respe
32、ctively; otherwise, penalties, to ensure production safety. 8.4.4.5 construction site safety protection 1) temporary facilities security protection a. construction of establishing traffic signs and lighting, vehicle and pedestrian traffic safety. B. underground structure within the construction site, the underground pipeline,0.1M磷酸盐缓冲液PH7.4 0.1 0.1 0.1 0.1 0.1 0.1 相当于谷丙转氨酶单位 0 28 57 97 150 200 相当于谷草转氨酶单位 0 24 61 114 190 每个样做3个平行，置37水浴30分钟，各管加2，4-二硝基苯肼液0.5mL
